Figure 7.
IRF-3 interaction translocates Bax to the mitochondria. (A) HT1080-siIRF-3 (siIRF-3) or IRF-3-restored cells (IRF-3) were transfected with poly(I:C); the mitochondrial and the cytosolic fractions were analysed by western blot for the indicated proteins. (B) Wt and IRF-3 KO MEFs were transfected with poly(I:C); the mitochondrial fractions and the cell lysates were analysed by western blot. (C) HT1080-siRIF-3 cells expressing IRF-3 were transfected with poly(I:C); the mitochondrial and cytosolic fractions were isolated and analysed as indicated.