Figure 5.
Effects of integrin blocking on osteogenic differentiation after 2 weeks of differentiation. (A) PCR of expression of α2 integrin on nanofibrous matrix (N), flat (solid) films (S) and 0.1% gelatin coated tissue culture plastic (C); (B) quantitiative PCR of expression of Runx2 on nanofibrous matrix (Nano), on nanofibrous matrix with control IGG isotype (Nano with isotype control), on nanofibrous matrix with mouse anti-human integrin alpha 2 monoclonal antibody (α2 blocking nano), on flat films (Solid), on flat films with control IGG isotype (solid with isotype control), and on flat films with mouse anti-human integrin alpha 2 monoclonal antibody (α2 blocking solid). * denotes p-value <0.05. ** denotes p-value <0.01. (C) quantitiative PCR of expression of osteocalcin on nanofibrous matrix (Nano), on nanofibrous matrix with control IGG isotype (Nano with isotype control), on nanofibrous matrix with mouse anti-human integrin alpha 2 monoclonal antibody (α2 blocking nano), on flat films (Solid), on flat films with control IGG isotype (solid with isotype control), and on flat films with mouse anti-human integrin alpha 2 monoclonal antibody (α2 blocking solid). * denotes p-value <0.05. ** denotes p-value <0.01.