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. Author manuscript; available in PMC: 2010 May 24.
Published in final edited form as: Nat Struct Mol Biol. 2009 May 10;16(6):616–623. doi: 10.1038/nsmb.1601

Figure 5. urg1 mRNA is more stable in cells lacking Cid1.

Figure 5

(a-c) Northern blots of urg1 transcript remaining at various time-points after uracil washout (top panel) in WT (a), cid1∆ (b) and ccr4∆ cells (c). urg1 levels were normalized to pik1 mRNA (bottom panel). The percentage of urg1 remaining at each time point (shown below the bottom panel) was calculated by comparison to the normalized amount at 0 minutes. (d) The percentage of urg1 mRNA remaining after uracil wash-out is shown for three strains, WT (open circles), ccr4∆ (black squares) and cid1∆ (open triangles). At least three independent replicates were performed; error bars represent SEM. (e) The half-life of urg1 mRNA in different strains is shown. At least two independent replicates were performed for each strain. * denotes p<0.01; ** denotes p<0.001. Error bars denote standard deviation.