Fig. 7. Keratocan promoter activity assay performed in Lum–/– animals measuring the ability of the keratocan promoter to drive β-GEO expression in the presence or absence of lumican.
The ability of an empty vector (Vector) or a lumican-expressing plasmid DNA construct (Lumican) to influence the expression of a β-GEO reporter gene by the keratocan promoter was measured 4 days after intrastromal injection. The presence of lumican significantly increases the activity of the keratocan promoter as shown in β-galactosidase 10–6 units/cornea as compared with vector control.