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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 Apr 26;184(11):6007–6013. doi: 10.4049/jimmunol.1000019

Figure 3.

Figure 3

Interaction with B7 is required for TCR-specific Treg cell development. (A) Diminished TCR transgenic Treg cell development in B7 KO mice. Total thymocytes from G113 TCR transgenic Foxp3gfp Rag1−/−, which are largely devoid of Treg cells, were mixed with Thy1.1+ filler cells, labeled with DDAO-SE and transferred intrathymically into WT, CD80−/− CD86−/− (B7 KO), or CD28−/− (CD28 KO) recipients. Expression of Foxp3 by DDAO+Vβ6+Thy1.1− donor cells was analyzed by flow cytometry at the indicated time. Each dot represents data from an individual mouse and the bar the average frequency. Data are pooled from at least two independent experiments (total mice: n=4, d1 WT and B7 KO; n=5, d2 WT; n=4, d2 B7 KO; n=13, d3 WT; n=10, d3 B7 KO; n=6, d3 CD28 KO). ** p<0.01.