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Tubulin polymerization followed by turbidity. 6 μm tubulin was incubated with 1 mm GTP at 37 °C and various concentrations of the NORE1 RBD alone (A), the NORE1 RBD and activated Ras (B), or control RBDs (C). The absorbance was detected at 350 nm. Error bars indicate the variation in turbidity of each experiment, which was performed at least three times.
