Figure 5.
Single injection of 1 μL elevates IOP in mice. (A) Daily IOP measurements for individual mice (n = 6) after a single 1-μL unilateral injection of microbeads (filled circles) and an equivalent volume of saline (open circles) in the opposing eye. Eyes were monitored for 23 days after injection without a significant decrease in IOP. Day 0 is before injection and day 1 is 24 hours after injection. Line represents IOP averaged across the cohort (mean ± SD). (B) A second cohort of mice (n = 8) with same injection regimen as in (A) but with a different experimenter. IOP returned to baseline by day 21. (C) Difference in mean IOP between microbead- and saline-injected mouse eyes (test-control) for the cohorts shown in (A) and (B) plotted against exposure time. (D) Cumulative exposure to elevated IOP over time (mm Hg per day) calculated from the difference curves in (C) with best-fitting multiparameter sigmoidal function (solid lines). Bracket indicates period without additional accumulation for the cohort in (B).