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. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 31;379(2):460–465. doi: 10.1016/j.bbrc.2008.12.118

Fig. 1.

Fig. 1

Down-regulation of flotillin-1 expression decreases caveolin-1 level in intestinal epithelial cells. SK-CO15 cells were transfected with flotillin-1, flotillin-2, caveolin-1 or control siRNAs and expression of lipid raft-resident proteins in these cells was analyzed by immunoblotting 72 h post-transfection. Representative immunoblots (A and C) and densitometric quantification of immunoreactive bands (B) are shown. Note dramatic down-regulation of caveolin-1 expression in flotillin-1 but not flotillin-2-depleted cells and lack of effect of caveolin-1 knock-down on flotillin isoforms level. Data presented as means ± SE (n = 5), *P < 0.05 compared to the control siRNA-treated group.