Fig. 7.
Gpx3 in kidney basement membranes. A: phase-contrast photomicrograph of kidney basement membrane-enriched fraction showing phase lucent sheetlike structures. Insets show that these structures are intensely immunostained (red) by anti-collagen IV (α-Col IV) and anti-laminin (α-laminin). B and C: matched phase-contrast and fluorescence images of basement membrane from Gpx3+/+kidney immunostained with anti-Gpx3 (red). D and E: phase-contrast and fluorescence images of Gpx3−/− kidney basement membrane immunostained with anti-Gpx3. No Gpx3 is detected. F and G: phase-contrast and fluorescence images of Gpx3−/− kidney basement membrane that had been incubated with purified Gpx3 before immunostaining with anti-Gpx3. Gpx3 binding to the basement membrane is evident.