Fig. 3.
PPARγ agonists induce the interaction of PPARγ with its binding protein PBP. (A) Schematic representation of the mammalian two-hybrid system used in this study. (B) The reporter plasmid UAS-TK-Luc was transfected into HEK293 cells with a VP16-PPARγ expression plasmid, either alone or with VP16-PPARγ and Gal4-PBP. Transfected cells were treated with different PPARγ activators (5 μM each) as indicated. Cells were harvested 24 h after treatment, and then luciferase activities were determined.