FIGURE 8.
AMPKα1 depletion increases the cleavage of caspase-3 and PARP in endothelial cells exposed to OGD for 4 h. A, AMPKα1−/− depletion, but not AMPKα2−/−, increases caspase-3 cleavage in isolated MAEC with/without OGD (4 h). Caspase-3 cleavage is reported as % of control ± S.E. β-Actin served as the respective loading controls (n = 3). ♣, p < 0.05 WT with OGD versus WT without OGD; †, p < 0.05 AMPKα1-KO with OGD versus WT with OGD; ‡, p < 0.05 AMPKα1-KO with OGD versus AMPKα2-KO with OGD; n = 3. B, effects of AMPKα1−/− depletion and AMPKα2−/− on OGD-induced PARP cleavage in isolated MAEC with or without OGD (4 h) are shown. Histone 3 is used as a control for nuclear protein. The blot is representative of three blots obtained from three individual experiments. C, shown are the effects of AMPKα1−/− depletion and AMPKα2−/− on OGD-induced apoptosis in isolated MAEC with/without OGD (4 h). Data are reported as % of TUNEL-positive cells in a microscopic field. ♣, p < 0.05 versus control; †, p < 0.05 WT with OGD versus AMPKα1−/− with OGD; n = 5.