Table 3. The affinity of hparp486 for blunt, nicked and 3’-extension DNA.
The percent fluorescence quenched, affinity (Kd) and Hill coefficient determined from the interaction of hparp486 with fluorescently labeled blunt ended, nicked or 3’-extension DNA are listed. The DNA constructs were either labeled in Position A or B, as illustrated in Figure 3B. Because of its pseudo symmetry, the 30Blunt construct was only labeled in one location.
| DNA Type | Fluorescence Quenched |
Affinity (Kd) | Hill Coeff. |
|---|---|---|---|
| 30Blunt | 33% | 2.82×10−7±4.0×10−8 | 0.9206 |
| 30Nick (pos. B) | 44% | 1.46×10−7±2.0×10−8 | 0.9670 |
| 30Nick (pos. A) | 8% | ---- | ---- |
| 30Ext (pos. B) | 35% | 1.30×10−7±1.3×10 −8 | 0.9904 |
| 30Ext (pos. A) | 19% | 6.80×10 −8 ±6.0×10−9 | 0.9802 |