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. Author manuscript; available in PMC: 2010 May 4.
Published in final edited form as: Stem Cells. 2007 Nov 29;26(2):517–524. doi: 10.1634/stemcells.2007-0016

Figure 2. Cell proliferation assay of labeled CD34+ cells.

Figure 2

Cultured Fe[750]+CD34+ cells were labeled with the membrane dye PKH26. (A) Flow cytometry analysis gating on the emission of PKH26 shows the number of cell divisions at 72 h. (B-D) Individual histograms of cells having undergone zero (16.9%), one (56.3%), or two (25.8%) cell divisions were further analyzed by CD34 expression and Fe[750] signal, to demonstrate that uptake in the undivided population was at least as high as is in the fractions that had undergone division.