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. Author manuscript; available in PMC: 2011 Apr 30.
Published in final edited form as: Circ Res. 2010 Mar 11;106(8):1394–1403. doi: 10.1161/CIRCRESAHA.109.210286

Figure 7. Iloprost reduces PAK activation in areas of disturbed flow in vivo.

Figure 7

(A) C57Bl/6J mice at 36–38 weeks received intraperitoneal injection of iloprost (20 μg; ~1 mg/kg) or saline control. After 2.5 or 24 hours, carotid arteries were collected and analyzed for PAK phosphorylation (Ser141), ICAM-1 expression, and VCAM-1 expression by immunohistochemistry. 40X images show the atheroprone region at the outer wall of the carotid sinus. 10X images show the entire artery (insets). (B,C,D) The percent of the vessel lumen staining positive for (B) phosphorylated PAK, (C) ICAM-1, and (D) VCAM-1 was determined. n = 3. * p < 0.05 by one-way ANOVA.