Figure 5.
Summary of chimera 2 mutagenesis constructs. (a) Left, motifs of HBII-180C snoRNA and mutated regions for each plasmid; right, plasmid name and mutated position (b) Quantification of knockdown level for different snoRNA constructs in transfected cells. The ratio of GFP-SMN fluorescence signals between transfected cells/nontransfected cells was calculated using SoftWoRx image processing software (Applied Precision). Graph shows average ratio between each mutant construct; error bars, SD; n > 10.