Fig. 2.
One-dimensional SDS-PAGE analysis of AG-modified proteins in presence and absence of farnesyltransferase inhibitors BMS-214,662 and L-778,123. AG labeling of cellular proteins in different myeloid leukemia cell lines was accomplished as described under “Experimental Procedures.” Equal amounts of total cellular proteins were separated by 15% SDS-PAGE and analyzed by immunoblotting using polyAG-Ab serum. α-N-Ras was used to demonstrate cell recovery from lovastatin treatment as well as FTI activity (u, unprenylated form; p, prenylated form), and α-tubulin was used to control for equal loading. Representative immunoblots of two separate experiments (n = 2) are shown. The use of each reagent (AGPP, BMS-214,662, or L-778,123) is indicated by a “+”.