FIGURE 6.
Molecular analysis of the preischemic Hif-p4h-2gt/gt hearts. Top and middle panels, Q-PCR analysis of the mRNA levels of Hif-1α, Hif-2α, Hif-p4h-1 and 3, and certain HIF target and other genes in adult preischemic Hif-p4h-2gt/gt hearts relative to age- and gender-matched wild-type hearts (n = 6). Hk-1 and -2, hexokinase-1 and -2; Gck-1, glucokinase-1; Pfkl, phosphofructokinase-L; Aldoa, aldolase A; Tpi, triose phosphate isomerase; Pgk-1, phosphoglycerate kinase-1; Eno-1, enolase-1; Ldha, lactate dehydrogenase A; A2bar, adenosine receptor A2b; A3ar, adenosine receptor A3; Adm, adrenomedullin; Hmox1, heme oxygenase 1; Mb, myoglobin; Apln, apelin; Pai-1, plasminogen activator inhibitor-1; Lox, lysyl oxidase. *, p < 0.05; **, p < 0.01; ***, p < 0.001. The error bars indicate S.D. Bottom panels, Western blotting of total protein extracts from preischemic wild-type (wt) and Hif-p4h-2gt/gt (gt/gt) heart was performed with the antibodies indicated.