FIGURE 3.
Analysis of wild-type Hif-p4h-2 mRNA levels and Hif-p4h-2, Hif-1α, and Hif-2α protein levels in selected tissues. A, wild-type Hif-p4h-2 mRNA levels in various Hif-p4h-2gt/gt tissues relative to wild-type tissues were analyzed by Q-PCR with primers amplifying the wild-type cDNA. A significant decrease (p < 0.001, n ≥ 5) in the Hif-p4h-2 mRNA level was seen in all Hif-p4h-2gt/gt tissues studied relative to the wild type, except in the liver. The error bars indicate S.D. B and C, Western blotting of total protein extracts from wild-type (wt) and Hif-p4h-2gt/gt (gt/gt) heart, skeletal (Sk) muscle, and kidney was performed with the antibodies indicated. Tubulin and HIF-1β stainings were used as loading controls.