FIGURE 2.
Analysis of the expression of Hif-p4h-2 in embryos and adult tissues. Hif-p4h-2 promoter-driven β-galactosidase expression was analyzed by X-gal staining of whole mounts or 5-μm paraffin sections of wild-type (wt), Hif-p4h-2+/gt (wt/gt), and Hif-p4h-2gt/gt (gt/gt) embryos and different tissues from adult mice. Embryonic day 12.5 Hif-p4h-2+/gt and Hif-p4h-2gt/gt embryos (A) and whole mount hearts (B) show ubiquitous X-gal staining with genotype-dependent intensity. C–F, histological analysis demonstrates pronounced X-gal staining in the cardiomyocytes of the Hif-p4h-2gt/gt hearts (C), in the smooth muscle cells of Hif-p4h-2gt/gt pulmonary arterial walls (D), and in myocytes of skeletal muscle (E), whereas no staining was observed in wild-type skeletal muscle (F).