FIGURE 2.
iPSCs from UMCs and AMCs display hESC characteristics. A, phase contrast captures of H9 human ESCs and iPSCs from UMCs (C1) and AMCs (C28) grown on either feeders or Matrigel layers; iPSCs at passage 10 or more are shown. The same cell lines were stained for alkaline phosphatase. Immunofluorescence microscopy also shows activation of the endogenous ESC program. A representative experiment is shown; scale bar corresponds to 150 μm. B, qPCRs of a representative experiment showing high levels of expression of ESC-specific transcription factors in iPSCs (passage 10 or more) from UMCs and AMCs compared with H9 ESCs and the donor cells. C, qPCRs showing silencing of the exogenous transgenes in all iPSCs relative to SKOM infected donor cells extracted at day 6 (at the time of splitting on feeder). D, qPCR for the indicated genes in UMCs, AMCs, and the human fibroblast cell line IMR90.