Fig. 1.
A subset of C. elegans nucleoporins is required for P granule integrity. (A-H) Single-plane confocal sections of 13- to 28-cell stage embryos derived from mothers treated with the indicated RNAi, and co-stained with antibodies against nuclear pore proteins (mAb414, red) and PGL-1 (K76, green). Embryos are oriented with anterior to the left and posterior to the right in this and all subsequent figures. P granules are perinuclear in control (empty vector), npp-1(RNAi), npp-19(RNAi) and lmn-1(RNAi), but mislocalized after npp-7(RNAi), npp-8(RNAi), npp-9(RNAi) and npp-10(RNAi). (I) Comparison of the percentage of embryos expressing the zygotic transgene pes-10::GFP and those with wild-type P granules following the indicated RNAi treatments (error bars are s.e.m.). *, P<0.01 (Student's t-test): a statistically significant reduction in the number of pes-10::GFP-positive embryos in RNAi versus control. The number of embryos scored (n) is shown below each bar.