FIGURE 7.
Chemokine production by Tc17 effectors. A, Tc17 cells produce chemokines involved in the attraction of Th1 cells. Four-day in vitro generated Tc17 OT-1 cells were stimulated with SIINFEKL peptide for 3 h in RPMI media containing brefeldin A. CD8 T cells were stained with Abs specific for CXCL9, CXCL10, CCL5, CCL3, and CCL4 and analyzed by FACs. B, Chemotactic activity of chemokines produced by Tc17 cells evaluated by chemotaxis assays. Tc17 cells or Tc17 and blocking Abs against the indicated chemokines were put in the bottom of a chemotaxis chamber. The 106 Th1 cells were deposited in the top chamber, and the Th1 cells that migrated to the bottom chamber were enumerated by FACS analysis after 1 h of incubation. The CI was calculated for all groups (see Materials and Methods).