Figure 1.
Depletion of FBXW7 increases mTOR and p-mTOR levels. (A) The levels of mTor and p-mTor, as well as the downstream mTor target S6-kinase (P-S6), are upregulated in Fbxw7−/− MEFs, whereas the levels of Akt and p-Akt (lower panels) were not appreciably affected. Two independent preparations were used. (B) Elevation of mTor in thymus (left panel) and spleen (right panel) from Fbxw7+/− mice. Lanes 1–3 contain extracts from wild-type, Pten+/−, and Fbxw7+/− mice respectively. (C) Overexpression of either full length FBXW7 (HA-FBXW7) or of a dominant-negative form (HA-FBXW7-ΔF) in 293T cells respectively decreases (lane 2) or increases (lane 3) the mTOR and p-mTOR levels..