Figure 4.
Capacity of CD45− side population (SP) cells to generate differentiated epithelium. CD45− SP cells seeded at 1 × 102 cells in transwell insert (0.45 µm pore, 12 mm diameter) were able to form a stratified epithelium in air-liquid-interface culture. Differentiation of CD45− SP cells was confirmed by immunostaining with antibodies for basal cell markers ΔNp63 (A), cytokeratin-5 (B), cytokeratin-18 (C), Muc5AC (D), tissue factor (E), and CD151 (F), and the staining was compared with patient matched airway sections (G–L).
