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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Immunol. 2010 Mar 3;184(7):3841–3849. doi: 10.4049/jimmunol.0902281

Figure 8. Reduced frequency of CD49a+ effector cells express apoptotic markers in the BAL.

Figure 8

A–C) At day 26 post-infection, BAL and SPL cells were analyzed by flow cytometry for Annexin V and 7AAD. Cells were gated by CD44hi CD4+ and then segregated by CD49a expression (A). Fig. A shows the proportion of CD49a+ (black bars) or CD49a− (white bars) cells that were Annexin V+ 7AAD+. B–C) CD44hi CD4+ cells on day 26 were further segregated by CD62L expression and analyzed for apoptotic marker expression on CD49a+ (black bars) and CD49a− (white bars) cells. Data are +/− SEM of n 4 per group; *=p<0.05 comparing CD49a+ and CD49a− groups. D–E) Two weeks post-infection, BAL and spleen cells were stained for Bcl-2 expression. D) Representative histograms for Bcl-2 staining on CD62Llo (dashed line), CD49a+ (gray filled), and CD49a− (solid line) subsets gated on CD44hi CD4+ cells from BAL (top) or spleen (bottom). E) Bcl-2 MFI was determined for CD49a+ and CD49a− subsets in isotype (Iso) or Bcl-2 antibody (Ab) staining conditions. Each point represents one mouse, with the average displayed as a horizontal line.