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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: Dev Dyn. 2009 Jan;238(1):86–99. doi: 10.1002/dvdy.21821

Figure 6. Leukocyte recruitment into the body of fad24hi1019 mutants.

Figure 6

A-C are WT and A′-C′ are fad24hi1019 mutant larvae. Immunolabeling for MPO (A,A′) and L-plastin (B,B′). (C,C′) Overlay of MPO and L-plastin co-immunolabeling shows that neutrophils, MPO+ and L-plastin+ (arrows), and macrophages, L-plastin+ and MPO- cells (arrowhead), contribute to the inflammatory response. (D) Confocal projections show the more elongated morphology of macrophages (arrowhead) compared to neutrophils (arrow). (E,E′) Whole-mount in situ hybridization for c-fms, a macrophage marker, red box indicates the CHT, and red arrows indicate macrophages in the body of fad24hi1019 mutants. Black arrows point to c-fms expression in the neural crest. (F,F′) XZ projections of MPO labeled WT and fad24hi1019 larvae. (G,G′) ZY projections of MPO (green) and p63 (red, to mark the epidermis) double-immunolabeled larvae, white line indicates the midline of the larva. (H) A 3.9 μm optical longitudinal section through the muscle of a fad24hi1019 larvae immunolabeled for MPO. All images except F,F′G,G′ are in lateral view, anterior to the left and all images are at 3 dpf. Bars = 200 μm. Representative results from three experiments.