Figure 5. Fad24hi1019 mutants exhibit defects in lipid metabolism.

(A, A′) Oil red O staining in a PTU treated WT (A) and fad24hi1019 (A′) larvae, arrows show areas of staining in the head and heart, and arrowheads show areas of staining in the vasculature that are absent in the fad24hi1019 mutant. (B, C) Oil red O staining in the body of control (B) or zfad24 init. MO (C) injected larvae. h = heart, isv = intersegmental vessel, da = dorsal aorta and dlav = dorsal longitudinal anastomotic vessel. Lateral view, anterior to the left at 3 dpf. Bars = 200 μm. Representative results from three experiments with greater than 10 larvae per condition per experiment.