FIGURE 1.
Distribution of Myo10 in mature osteoclasts. A, mouse bone marrow-derived osteoclasts were plated on glass and examined by confocal microscopy for the localization of Myo10 (green) and F-actin (red). Arrows indicate regions of Myo10 enrichment near the podosome belt. Scale bars, 10 μm. B, images of RAW264.7-derived osteoclasts were prepared as in A. C, osteoclasts were plated on thinly cut ivory and examined by confocal microscopy to view Myo10 (green) in relation to the actin ring/sealing zone (F-actin, red). Myo10 is completely absent in the sealing zone in both RAW264.7 and MBM derived osteoclasts. Z-stack images of an MBM-derived osteoclast are shown to illustrate Myo10 distribution throughout the cell. Scale bars, 20 μm.