Fig. 2.
Crystal structure of the μ4 C-terminal domain in complex with a peptide signal from APP. (A) Ribbon representation of human μ4 C-terminal domain with subdomain A in blue, subdomain B in red, and the APP peptide (TYKFFEQ; stick model) in yellow. (B) Superposition of μ4 and rat μ2 (with the EGF receptor peptide in magenta; pdb entry 1BW8, Owen and Evans, 1998). In orange (A) is show n a loop that is disordered in μ2. The position of the N- (N, Nμ2 and Nμ4) and C-termini (C) are indicated. (C and D) Model of the AP-4 (C) and structure of the AP-2 core (D; Collins et al. 2002; pdb entry 1GW5) core complexes showing the position of the respective peptide-binding sites and the PIP2–binding site on the AP-2 α subunit. See also Fig. S1.