Fig. 2.
Enhancer reporter assay. (A) The enhancers were cloned in front of a Myc promoter-driven or TK promoter-driven luciferase-GFP reporter. (B) Luciferase activity of enhancer-Myc-luciferase constructs in prostate cell line LnCAP, PC3, and colorectal cell line SW620. (C) Luciferase activity of enhancer-TK-luciferase constructs in prostate cell line LnCAP, PC3, and colorectal cell line SW620. Enhancer reporter plasmids were cotransfected into cell lines as described in Materials and Methods. Luciferase activity was measured 24 h after transfection by using the Dual-Glo Luciferae assay system.