Fig. 1.
(A) Diagram of Xho1-Xho1 targeting construct, containing Pex7 exons 1–3, and genomic sequence flanking the targeting construct. Neor is inserted at AflII-NheI sites, removing 10 bps of intron 2. EcoR1 sites and probes used for the diagnostic southern digests are shown. (B) ES clone 1 shows correct position of the targeting construct by southern analysis. On the left, the 5′probe detects a 5.1 kb fragment; center, 3′ probe detects a 3.9 kb fragment. Right, both probes detect the correct fragment in tail gDNA, verifying germline transmission (only 3′ probe is shown). L, ladder.