FIGURE 7.
Purified exosomes containing ULBP3 are potent modulators of NK cell NKG2D-mediated cytotoxicity. A, primary NK cells recognize CHO-MICA transfectants in an NKG2D-dependent fashion. B, effect of purified exosomes on NK cell cytotoxicity against CHO-MICA and untransfected CHO. Purified exosomes from either untransfected or ULBP3-transfected CHO cells (40–100 ng total protein) were incubated with primary NK cells for 24 h. After preincubation with exosomes, NK cells were used as effectors in a cytotoxicity assay (measured using a fluorimetric assay). E:T, effector:target ratio.