Figure 6. TNFR1 signaling is required for obesity-induced tumor promotion.
(A). Livers of WT or TNFR1 deficient male mice kept on LFD or HFD after DEN administration (25 mg/kg) at 14 days of age.
(B). Tumor multiplicity and maximal sizes in DEN-administered male mice of the indicated genotypes kept on either LFD □ or HFD ■ (n=10-13).
(C). Body weight gain during tumor development in WT or TNFR1-/- male mice kept on LFD or HFD after DEN administration.
(D). Non-tumor (NT) liver and HCC (T) from the mice described in (A) were isolated, lysed, gel separated and the phosphorylation states of the indicated proteins were examined by immunoblotting. Shown are the results for two separate HCCs per condition.