Fig. 1.
hsa-miR-26a is a component of the 12q13.3–14.1 amplicon. (A) Schematic map of 12q13.3–14.1 amplifications in the 32 GBM specimens (y axis), including the genomic locations of CENTG1, CDK4, hsa-miR-26a, and CTDSP2. The thickness of each horizontal line indicates the degree of amplification. (B) Scatter plot of DNA copy number versus mRNA expression for CTDSP2. The Pearson’s correlation coefficient is 0.83. (C) Scatter plot of DNA copy number versus microRNA expression values for hsa-miR-26a. Each circle represents the expression value from a single GBM specimen. The horizontal dotted line represents the median miR-26a expression value of the samples in which the DNA segment was neither amplified nor deleted (−0.2 < segment value < 0.2). The solid line shows a linear fit of data. Pearson’s correlation coefficient is 0.72. (D) Correlations between miR-26a and mRNA expression profiles identified using the method described in the text. Gray areas represent data not used for correlation computations to filter extraneous effects such as copy-number alterations and DNA methylation.