Figure 3.
Effects of PEDF-derived peptides on vessel sprouting. (A) Representative photographs of vessel sprouting in the presence and absence of 10 nM peptide 34-mer. (B) Vessel sprouting assays with 1-, 10-, and 100-nM concentrations of PEDF-derived peptides (x-axis). Negative control was without ECGS; positive control was with ECGS and without peptides. Additions to rings with ECGS are indicated in the x-axis. The relative area of vessel sprouting (y-axis) as a function of peptide concentration was plotted. Each bar corresponds to the average of four replicate assays ± SD.