TABLE 1.
Chronic ethanol treatment produces a reversible increase in NMDAR cluster size and density
Cluster size and density expressed as mean ± S.E.M. Student's t test; n > 8 coverslips for each condition.
| Cluster Size (Pixels/Cluster) |
Cluster Density (Clusters/10 μm) |
|||||
|---|---|---|---|---|---|---|
| Control | EtOH | WD 4 h | Control | EtOH | WD 4 h | |
| NR1 | 6.76 ± 0.4 | 12.15 ± 0.5* | 6.86 ± 0.6† | 5.90 ± 0.4 | 9.17 ± 0.4* | 5.56 ± 0.8† |
| syp | 13.70 ± 0.8 | 14.14 ± 1.0 | 14.26 ± 0.7 | 11.06 ± 0.4 | 12.39 ± 0.8 | 12.02 ± 0.7 |
| NR2B | 7.51 ± 0.3 | 17.25 ± 1.1* | 9.28 ± 0.4† | 5.46 ± 0.3 | 8.59 ± 0.6* | 4.82 ± 0.6† |
| PSD-95 | 11.54 ± 0.6 | 11.28 ± 0.5 | 10.45 ± 0.8 | 12.05 ± 1.0 | 11.99 ± 0.9 | 9.92 ± 0.9 |
| GluR1 | 12.6 ± 0.9 | 12.8 ± 0.8 | 12.8 ± 1.0 | 7.44 ± 0.6 | 6.84 ± 0.9 | 6.62 ± 0.9 |
EtOH, chronic ethanol exposure; WD, withdrawal.
*
p < 0.01 compared with untreated control.
†
p < 0.01 compared with chronic ethanol treatment.