Figure 1.
In vitro characteristics of bone marrow–derived rat multipotent mesenchymal stroma cells (MSCs). (a) Spindle-shaped morphology of MSCs generated from adult bone marrow. Cultures were stained with crystal violet. (b) Differentiation capacity of MSCs into adipocytes and (c) osteoblasts was assessed for passage 2 cells. (b) Lipid vacuoles were stained with Oil-red-O. (c) Osteogenic differentiation was detected by staining with Alizarin Red. Bar = 60 µm. (d) Flow cytometric analysis of MSCs using antibodies against CD73 (left panel), CD90 (middle panel), and the hematopoietic marker CD45 (right panel).