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. Author manuscript; available in PMC: 2010 Mar 8.
Published in final edited form as: Nat Immunol. 2008 Jul 11;9(8):847–856. doi: 10.1038/ni.1631

Fig. 1. Silica induces release of mature IL-1β and activated caspase-1 in human PBMCs in a caspase-1 dependent manner.

Fig. 1

(a) Human PBMCs were primed with LPS (25 pg/ml) or left untreated for 3h and subsequently stimulated with silica crystals or controls. After 6h, supernatants were assessed for IL-1β production by ELISA and Western blot. ELISA data of four independent donors are depicted (upper panel) and Western blot analysis of one representative donor is shown (lower panel). (b) LPS-primed human PBMCs were stimulated with either silica crystals, MSU crystals, ATP or transfected with dAdT. 6h after stimulation, supernatants were analyzed for IL-1β by ELISA and assessed for matured IL-1β or activated caspase-1 by Western blot. Data of one representative donor out of three are depicted. (c) Human LPS-primed PBMCs were stimulated with silica crystals in the presence or absence of the caspase-1 inhibitor z-YVAD (10 µM). After 6h, supernatants were assessed for IL-1β by ELISA and Western blot. Mean values (+SD) of two donors are depicted as fold increase (ELISA) and Western blot data of one representative donor are shown.