FIG. 3.
Preconditioning of MSCs induced canonical activation of NF-κB. Immunoblots from the protein samples collected from the cells harvested 4 h after their respective treatment. Immunodetection with respective antibodies showed phospho-NF-κB (p65) in (A) cytoplasmic and (B) nuclear protein fraction in Non-PCMSCs, PCMSCs, PCMSCs treated with Wt and PCMSCs treated with NBD. (C) TransAM assay showing activity of phospho-NF-κB (p65) in the nuclear fraction in the same samples. (D) Immunoblot showing phospho-NF-κB (p65) in the nuclear protein fraction from Non-PCMSCs, PCMSCs, and PCMSCs transfected with NF-κB (p50) siRNA and PCMSCs transfected with scrambled siRNA. (E) TransAM assay showing activity of NF-κB (p65) in the nuclear fraction from different groups used in gene silencing studies. The densitometry (IDV) in all the blots was determined by using β-actin (Octamer-1 for nuclear fraction) as internal control.