FIG. 2.
Preconditioning induced expression of pro-survival proteins. Immunoblots from the protein samples collected from the cells harvested 4 h after their respective treatment. Immunodetection with respective antibody showed significantly increased phosphorylation of (A) PI3K, (B) Akt, and (C) GSK3β in Non-PCMSCs, PCMSCs, and PCMSCs treated with Wt. The densitometry was done by using β-actin as internal control. (D) Immunoblot showing phospho-GSK3β from Akt kinase assay which used GSK3β as substrate.