Figure 2.
Biotinylated miR-20a is expressed and active. Flag-HA-AGO1 cells were transfected with biotinylated miR-20a, miR-125b or an irrelevant sequence (ir.) as indicated. (A) Levels of miR-20a were quantified using qRT-PCR (Log scale); representative of two independent experiments. (B) E2F-1 protein, a previously characterized miR-20a target (20), monitored by western blot 3 days after transfection; representative of 3 independent experiments. (C) E2F-1 mRNA monitored by qRT-PCR. Standard deviation is from 10 independent experiments. ***P < 0.001.