Figure 7.
Stable re-expression of Smad4 reduces tumorigenicity and metastasis of MC38 cells. (A) Subcutaneous tumors were generated by the clones and tumor volumes were measured. Growth curves for tumors are presented as the mean ±S.D. of five tumors. *P<0.03, **P<0.05, ***P<0.01 (B) Liver metastasis was examined 21 days after injecting MC38 cells/clones into spleens of C57BL/6 mice as described above. (C) The graph shows average of the ratio between metastatic liver weight and total body weight of mice in each group (n=4). *P <0.001. This experiment was repeated two times.