Table 1.
Relative Staining in Aged Mice with CCI Injury or Sham Operation
| Measure | Structure | Group | n | Contra:Ipsilateral1 | p value2 |
|---|---|---|---|---|---|
| T2 hypointensities3 | Thalamus | Sham | 3 | 1.014 ± 0.020 s.d. | 0.024 |
| CCI injured4 | 6 | 1.355 ± 0.134 s.d. | |||
| Internal Capsule | Sham | 3 | 1.116 ± 0.078 s.d. | 0.095 | |
| CCI injured 4 | 6 | 1.318 ± 0.190 s.d. | |||
| Iron histochemistry5 | Thalamus | Sham | 7 | 1.000 ± 0.024 s.d. | 0.001 |
| CCI injured 4 | 7 | 1.564 ± 0.142 s.d. | |||
| Internal Capsule | Sham | 7 | 0.960 ± 0.078 s.d. | 0.710 | |
| CCI injured | 7 | 0.945 ± 0.092 s.d. | |||
| Iba16 | Thalamus | Sham | 8 | 1.006 ± 0.007 s.d. | 0.015 |
| CCI injured 4 | 7 | 1.087 ± 0.056 s.d. | |||
| Internal Capsule | Sham | 8 | 0.995 ± 0.022 s.d. | 0.035 | |
| CCI injured 4 | 7 | 1.096 ± 0.045 s.d. | |||
| GFAP6 | Thalamus | Sham | 8 | 1.006 ± 0.023 s.d. | 0.035 |
| CCI injured 4 | 7 | 1.067 ± 0.033 s.d. | |||
| Internal Capsule | Sham | 8 | 1.010 ± 0.052 s.d. | 0.752 | |
| CCI injured | 7 | 1.057 ± 0.057 s.d. |
A ratio greater than 1 indicates that the ipsilateral structure was darker than the contralateral structure which is due to a greater hypointensity (MRI) or darker staining (iron histochemistry, Iba1, and GFAP) in the former structure.
Two-tailed Wilcoxon two sample test, comparing CCI injured with sham ratios.
Mean intensities measured from T2-weighted MR images in 0.244 mm2 square regions at center of ipsilateral hypointensity and at corresponding contralateral location.
Ipsilateral structure was significantly darker than the contralateral structure. The Wilcoxon matched-pairs signed-ranks test, significance p ≤ 0.05.
Mean brightness intensities of two areas (210 μm diameter circles) with maximal staining in the ipsilateral structure and two corresponding areas in the contralateral structure.
Mean brightness intensities of two areas (150 μm × 150 μm squares) with maximal staining in the ipsilateral structure and two corresponding areas in the contralateral structure.