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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: J Comp Neurol. 2008 Apr 10;507(5):1831–1845. doi: 10.1002/cne.21649

Fig. 6.

Fig. 6

CPG15-2 is a GPI anchored protein expressed in membrane-bound and soluble-secreted forms. A: CPG15-2 is a GPI-anchored membrane protein. HEK293 cells expressing FLAG-tagged CPG15 or CPG15-2 were immunostained with an anti-FLAG antibody (magenta) under nonpermeabilizing conditions. EGFP (green) coexpressed from the same vector marks the transfected cells. Both CPG15 and CPG15-2 show a punctate staining on the cell surface that is significantly reduced by phospholipase C treatment prior to immunostaining. B: Coexisting membrane-bound and soluble forms of CPG15 and CPG15-2. Cell lysate and culture supernatant from HEK293 cells stably transfected with FLAG-tagged cpg15, cpg15-2, or empty vector (—) were immunoprecipitated with an anti-FLAG antibody, then probed on a Western blot with an anti-FLAG antibody. PLC, phospholipase C; cell, cell lysate fraction; sup, culture supernatant fraction. Scale bar = 20 μm.