Fig. 4.
Activity changes of sc(C49 C62)2(F94)2Azo. sc(C49 C62)2(F94)2Azo was illuminated with blue light → UV light → blue light → UV light, interrupted by a long incubation in the dark. DNA cleavage activity was determined by a DNA cleavage assay (4 nM DNA, 12 nM enzyme) under illumination with blue or UV light and during incubation in the dark, respectively. On the Top the gel electrophoretic analysis is shown, and on the Bottom the plot of the absolute activities as determined in the respective illumination states is shown.