Fig. 6. Ionomycin stimulates TNFR-1 translocation to the plasma membrane.
MDCK cell lines over-expressing either WT or KD MLCK or parental (MDCK) cells were treated for 5 min with ionomycin or with vehicle (dimethyl sulfoxide (DMSO)). A, the levels of phosphate incorporation into myosin II RLC (mol Pi/mol RLC) were determined following urea-glycerol gel electrophoresis and densitometry of Western blots using anti-RLC antibody to detect RLC. B, the relative amounts of TNFR-1 in the biotin-labeled fraction were determined by Western blotting using anti-TNFR-1 antibody. The amount of TNFR-1 in the biotin-labeled fraction was also examined in the presence or absence of the MLCK inhibitor ML-7. The results shown represent the mean ± S.E. from four experiments.