Figure 6. Inhibition of Nrf2 in DU-145 cells decreases cell proliferation in vitro, and reduces tumor growth in vivo.
(A) NRF2 promotes prostate cancer cell proliferation. Fifty thousand cells were seeded in each well of 6-well plates and relative cell number was measured everyday for 5 days. * P<0.001. (B) DU-145-LucshRNA and DU-145-Nrf2shRNA cells were injected into the flanks of male athymic nude mice (n = 13 for LucshRNA cells group; n = 12 for Nrf2sRNA group). Weekly measurements of tumor size were conducted for 5 weeks, and the mean tumor volume was calculated. Weight of the tumor was recorded at the end of the experiment. Data were analyzed using 2-sample Wilcoxon rank-sum test (* U=424-604, P-value<0.001).