Figure 2. Increased Macrophage Recruitment 3 days Post-Th1 Infusion.
These photomicrographs are representative images of whole lung stained with anti-macrophage antibody, MAC-2 (a gift of Elaine Raines, University of Washington). Control lung represents untreated mice, Th1 lung represents Ly5a mice that have received allogeneic Th1-cells (anti-Ly5a) intravenously 3 days before harvest. Lungs have been fixed in 4% paraformaldehyde and paraffin-embedded. The primary antibody is rat anti-mouse MAC-2, the secondary antibody is biotin-labeled donkey anti-rat ab. Colorimetric detection was done with diaminobenzidine staining (Vector Lab) and the tissue counterstained with hematoxylin.