Fig. 3.
Cytoskeleton-based descriptors segment and identify individual osteoblastic cells within hMSCs cultures exposed to soluble factors. MDS, utilizing the morphometric descriptors to create three new dimensions (D1–D3) that each represent nonlinear combinations of the 43 descriptors, demonstrates that individual cells exposed to OS media (Blue) cluster separately from cells in either AD (Red) (A–C) or BA (Black) (D–F) media. All cells were cultured on fibronectin/glass at 3,000 cells per cm2. This segmentation was observed in at least three different cultures consisting of two donors (Donor 1 was a 36-year-old-old male (A, D, B, E); Donor 2 was a 22-year-old female (C, F) and two passages (2 (A, C, D, F) and 4 (B, E)). No segmentation was observed between the AD and BA media conditions (D–F). For (A–C) data is pooled from four different wells for each condition, in (D–F) data are pooled from two wells for each condition. (A–C) Support vector machine (SVM) classification of clustering shows that OS vs. AD classification can be achieved with high accuracy (ACC), sensitivity (Sens), and specificity (Spec). Error reported on SVM classification represents the standard deviation for ACC, Sens, and Spec for N = 50 twofold cross validation pseudoexperiments of MDS data plotted in (A–C).