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. Author manuscript; available in PMC: 2010 Jan 29.
Published in final edited form as: Exp Neurol. 2005 Mar;192(1):11. doi: 10.1016/j.expneurol.2004.05.038

Fig. 2. NG2+ polydendrocyte response to EB injection.

Fig. 2

(A) NG2+ cells are evenly spaced in the uninjured VLF and have irregularly shaped cell bodies. (B) At 2 dpi, NG2+ polydendrocytes demonstrate increased NG2+ staining and short processes. In (B, C, and E), the larger inset box shows the entire lesioned VLF while the inner box shows the region shown at higher magnification. (C) By 7 dpi, NG2+ polydendrocytes exhibited complex morphologies with many branching processes. (D) NG2 did not label activated macrophages at 7 dpi as evidenced by a lack of NG2 and ED-1 coexpression. (E) Increased NG2+ immunoreactivity is observed in EB lesions up to 28 dpi. (F) In animals sacrificed at 2 dpi, 85% of the polydendrocytes within the lesion express BrdU following a 24-h BrdU pulse on Day 2, suggesting that the increase in polydendrocyte density within EB lesions is at least partially due to local proliferation. (G) Polydendrocyte density within EB lesions is significantly increased at 2, 7, and 28 dpi compared to uninjured controls. Data are expressed as the mean ± SD (uninjured; n = 10, Day 2; n = 9, Day 7; n = 8, Day 28; n = 8). One-way ANOVA (F = 50.5, df = 3.31, P < 0.001). Tukey post hoc (all comparisons, *P < 0.001). Scale bar = 5 µm in (A, D, and F); 8 µm in (B); 3 µm in (C and E).